NR AWQV
AU Yan,Z.X.; Roth,K.; Stitz,L.; Gaedt,S.; Zenner,H.P.; Mauz,P.S.
TI Decontamination of thermolabile instruments from pathological prions
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions SA-15
PT Konferenz-Poster
AB Pathological prion proteins are known infectious agent for transmissible TSE, the PrPsc have been found to be distributed all over the lymphatic tissues, and in the blood as well. We have reported on a pathological prion infectivity model using hamsters and prion protein contaminated steel wires to demonstrate transmission and decontamination of infectious prion proteins. We showed that the hamster adapted pathological prion proteins could bind to the steel wire in dried form and cause disease after the contaminated wires are implanted or inserted only for a very short time. When the contaminated wires were treated with different cleaning, disinfection and/or sterilization procedures before implantation, infectivity was reduced which was manifested directly by a prolonged surviving time of the test animals. This model is very useful as a prion bioassay to validate reprocessing procedures for surgical instruments. Because the pathological prion proteins are highly resistant to most of the routine hospital sterilization procedures, reusable surgical instruments need to be regarded as a potential source of transmission. Delicate surgical instruments such as endoscopes need gentle sterilization procedures, but prion proteins are known to be very resistant toward convenient sterilization processes. Most of the processes described as being effective against pathological prion protein are a combination of highalkaline detergents and steam sterilization. In searching of low temperature sterilization processes, we also tested combinations of different cleaning disinfectants/detergents followed by a low temperature sterilization process, since a thorough cleaning of the instrument before sterilization is a critical step in decontamination. After several long-time bioassay studies, we found that washing with alkaline detergent followed by sterilizing in a hydrogen peroxide gas plasma sterilizer (Sterrad) to be the most effective lowtemperature, non-autoclaving reprocessing procedure against infectious prion proteins. All the animals have survived until the end of the test (18 months) without showing any clinical signs.
AD Z.X. Yan, K. Roth: SMP GmbH Pruefen Validieren Forschen; Paul-Ehrlich-Strasse 40; 72076 Tübingen, Germany; L. Stitz: Friedrich Loeffler Institut; Paul-Ehrlich-Strasse 28; 72076 Tübingen, Germany; S. Gaedt, H.-P. Zenner, P. Mauz: Hals Nasen Ohren Klinik der Eberhard-Karls Universität; Elfriede Aulhorn-Strasse 5; 72076 Tübingen, Germany
SP englisch
PO Italien