NR AWSK
AU Tkacikova,L.; Rolkova,G.; Bires,J.; Novak,P.; Kontsekova,E.; Imrichova,D.; Bhide,M.; Hanusovska,E.; Mikula,I.; Novak,M.
TI PrP gene polymorphism in sheep breeds in Slovakia and susceptibility to scrapie
QU Acta Virologica 2006; 50(4): 257-61
PT journal article; research support, non-u.s. gov't
AB We analyzed the prion protein (PrP) genotype based on the codons 136, 154 and 171 and assigned to five risk groups (R1-R5) in healthy and scrapie-affected sheep in Slovakia. In healthy (asymptomatic) population, 119 Merino, 106 Improved Valachian, 117 Tsigai, and 48 Suffolk breeds were tested. Among the asymptomatic sheep, the low-risk genotypes R1 and R2 were most abundant in Suffolk (94%) and Merino (84%) breeds, followed by Tsigai (58%) and Improved Valachian (40%) breeds. The medium-risk group R3 was most frequent in Improved Valachian (31%) breed, followed by Tsigai (21%), Merino (10%), and Suffolk (6%) breeds. The occurrence of high-risk groups R4 and R5 was none in Suffolk breed, followed by Merino (6%), Tsigai (21%), and Improved Valachian (30%) breeds. Since 2003, altogether 48 cases of scrapie have been confirmed in Tsigai (38), Merino (4), Improved Valachian (2), Improved Valachian x Tsigai (3), and Suffolk (1) breeds. Among sheep with scrapie, Merino breed belonged to the medium-risk group R3. The majority of scrapie-affected Tsigai sheep were classified into high-risk R5 (50%) and medium-risk R3 (42%) groups. We showed an association of scrapie with medium- and high-risk groups of PrP genotype in Slovakia. In particular, the glutamine at position 171 appears to be of major importance for the susceptibility to scrapie.
MH Animals; Codon/genetics; Electrophoresis, Polyacrylamide Gel; Genetic Predisposition to Disease; Genotype; Molecular Sequence Data; Nucleic Acid Denaturation; Polymerase Chain Reaction; *Polymorphism, Genetic; Prions/*genetics; Scrapie/*genetics; Sequence Analysis, DNA; Sheep/*genetics; Slovakia
AD University of Veterinary Medicine, Komenskeho 73, 04181 Kosice, Slovak Republic.
SP englisch
PO Slowakei