NR AWST
AU Romero Trevejo,J.L.; Pedrera,M.; Sanchez-Cordon,P.J.; Bautista,M.J.; Gomez-Villamandos,J.C.
TI Role of macrophages population on gut associated lymphoid tissues from mice inoculated with scrapie
QU European Society of Veterinary Pathology, 24th Meeting, Edinburgh, Scotland, 30.8.-2.9.2006, Poster 55: 164-5
IA http://www.esvp.eu/ESVP_meetings/Proceedings_2006.pdf
PT Konferenz-Poster
AB
To study the role, numerical and qualitative changes of macrophages population on gut associated lymphoid tissues, 102 C57BL mice were orally inoculated with the scrapie RML strain (CISA-INIA), while 30 animals with the same characteristics were maintained without inoculate and used as negative controls. Animals were slaughtered in batches of 6 from 15 to 400 days post-inoculation (dpi); samples from intestine and Peyer?s patches were fixed in 10% buffered formalin and zinc solution and embedded in paraffin-wax. Haematoxilin-eosin and avidin-biotin peroxidase complex techniques were used for structural and immunohistochemical studies.
Animals showed typical symptoms of the disease from 270 dpi, consisting in stagger, arched back and erected tail and hair. However, no gross or microscopical lesions were found in the necropsies or in the structural study. The immunohistochemical study carried out using the Rb486 antibody showed the presence of PrPres in tingible body macrophages and in dendritic cells of Peyer?s patches from 60 dpi, increasing the number of immunolabelled cells against this antibody until 180 dpi. To study the changes in macrophages population, Mac-3, anti-TNF?, anti-IL-1? and anti-IL-6 antibodies were used, and positive cells were counted and tested for significance (P<0.05) by Student?s t-test.
This work will allow to profundice in the knowledge of the pathogenesis of prionic diseases, considering that it has become increasingly apparent that immune system cells could participate in clearance of the infectious agent at entry sites of infection, supply routes for agent spread and provide fertile ground for agent replication or accumulation.
AD J. L. Romero Trevejo, M. Pedrera, P. J. Sánchez-Cordón, M. J. Bautista, J.C. Gómez-Villamandos, University of Córdoba, Spain
SP englisch
PO UK