NR AWSU
AU Chowdhury,E.H.; Marium,N.; Islam,A.; Rahman,M.M.
TI A slaughterhouse surveillance of bovine spongiform encephalopathy in Bangladesh
QU European Society of Veterinary Pathology, 24th Meeting, Edinburgh, Scotland, 30.8.-2.9.2006, Poster 59: 168
IA http://www.esvp.eu/ESVP_meetings/Proceedings_2006.pdf
PT Konferenz-Poster
AB Bovine spongiform encephalopathy (BSE) is a transmissible fatal neurodegenerative infectious disease first identified in the United Kingdom in 1987. This disease has a causal link with an old disease ?Scrapie? which has been prevalent in sheep for over 200 years. The principal component of the infectious agent responsible for the disease appears to be an abnormal isoform of the host encoded prion protein (PrP), designated ?PrPsc?. The emergence of variant Creutzfeldt Jakob Disease (vCJD) in humans in 1996 in UK and its causal links with BSE has highlighted the need for comprehensive study on its pathogenesis, diagnosis, prevention and eradication approaches. To control BSE within a country or to prevent the entry of BSE into a country, EU, USDA and few other countries have taken emergency measures in prevention, education, surveillance, and exportation of bovine origin materials (feeds, tallow, gelatin, etc) into these countries need BSE surveillance certificate. Bangladesh has no BSE surveillance so far. In this study, preliminary slaughterhouse surveillance is conducting in two districts, Dhaka and Mymensingh, in Bangladesh. Around 1000 brain samples from Cattle older than 30 months of age, slaughtered for human consumption in the district slaughterhouses, were collected for this study. The brainstems (obex), pyriform lobe, pieces of cerebellum and cerebrum were subjected for Histopathology, immunohistochemistry and ELISA using an anti-PrP monoclonal antibodies 6H4 (prionic AG, Switzerland) specifically reacting with epitopes on ruminant PrPsc and commercial immunoperoxidase and ELISA kit. Although some of the brainstems showed mild gliosis and inflammation, but none of the brainstems exhibited characteristic histopathologic lesions as found for BSE. No PrPsc was detected on these brainstems using immuohistochemistry and ELISA. From this study it apparently seems that BSE is not present in the native cattle in Bangladesh. Still samples are being collected from different regions and are analysing using above-mentioned methods.
AD E. H. Chowdhury, N. Marium, A. Islam, M. M. Rahman, Bangladesh Agricultural University, Bangladesh
SP englisch
PO UK