NR AWSW
AU del Carmen Ferreras,M.; Fuertes,M.; Garcia-Pariente,C.; Benavides,J.; Perez,V.; Garcia-Marin,J.F.
TI Immunohistochemical evaluation of the presence of PRPD in the intestine, ileocaecal lymph node and central nervous system of clinical and subclinical natural ovine scrapie cases
QU European Society of Veterinary Pathology, 24th Meeting, Edinburgh, Scotland, 30.8.-2.9.2006, Poster 65: 172-3
IA http://www.esvp.eu/ESVP_meetings/Proceedings_2006.pdf
PT Konferenz-Poster
AB Scrapie is a neurodegenerative disease of adult sheep characterized by the accumulation of the abnormal disease-specific prion protein (PrPd). The aim of this study was to investigate the distribution of PrPd in the gut associated lymphoid tissue -GALT-, enteric nervous system -ENS-, ileocaecal lymph node (ILN) and central nervous system (CNS) in clinical and subclinical Scrapie cases, and the relationship with the PrP genotype showed by the animals. Forty-six sheep from 3 flocks with confirmed cases of Scrapie have been studied. Animals were selected according to their age and genetic relationship with previous clinical cases, and the presence of clinical signs. PrP genotype was determined in 44 sheep. Immunohistochemical studies using 6H4 and L42 antibodies were performed in samples from the CNS, distal ileum, ileocaecal valve and ILN. PrPd positivity in CNS was confirmed in 23 cases, all with clinical signs, and in 15 of them, these lesions coexisted with the presence of PrPd in macrophages-like or dendritic-like cells in lymphoid follicles. Seven sheep showed positivity only in GALT and ILN. In 15 cases, both brain and digestive tissues were immunonegative. PrPd was detected in the ENS in 6 animals, and in 5 of them also in the GALT. Positive cases were more frequent in 2-4 years-old individuals, although one sheep younger than one-year-old, daughter of a clinical case, showed intestinal positivity. PrPd was found mainly in sheep with ARQ/ARQ (19) and ARQ/ARH genotypes (7). One sheep with ARR/ARR genotype presented immunopositivity in ILN and no signal was present in 3 ARQ/VRQ amimals. Four sheep suffered both Scrapie and Visna virus infection. PrP accumulations can be detected in the ENS and the GALT both in clinical and subclinically affected sheep. Digestive PrPd was detected in a Scrapie-resistant sheep carrying the PrPARR/ARR genotype, suggesting, as it has been pointed out, that genotype does not seem to affect the mechanisms of uptake of PrPd.
AD María del Carmen Ferreras, Miguel Fuertes, Carlos García-Pariente, Julio Benavides, Valentín Pérez, Juan Francisco García-Marín, Dpt. Patología Animal: Medicina Animal (Anatomía Patológica), Spain
SP englisch
PO UK