NR AWTA
AU Siso,S.; Gonzalez,L.; Martin,S.; Jeffrey,M.J.
TI The patterns of accumulation of prion protein during preclinical sheep scrapie and BSE suggest different pathways of neuroinvasion
QU European Society of Veterinary Pathology, 24th Meeting, Edinburgh, Scotland, 30.8.-2.9.2006, oral presentations, Poster 56: 165-6
IA http://www.esvp.eu/ESVP_meetings/Proceedings_2006.pdf
PT Konferenz-Poster
AB
Iatrogenic transmission by blood transfusion has been described from cases of human vCJD, experimental ovine BSE and natural sheep scrapie, demonstrating that blood in these prion diseases is infectious. However, comparative pathological features of the diseases in blood donors and recipients have not reported yet on the effect of blood exposure compared to the oral one. The present study describes the pathological phenotype of PrPd deposition in sheep recipients succumbing to clinical disease after blood transfusion from experimental ovine BSE or natural scrapie sheep donors.
Detailed immunohistochemical studies were carried out on brain and lymphoreticular tissues. Sheep challenged intravenously with BSE cattle brain homogenate were used as positive controls. Blood recipients were examined after they developed clinical signs or died from intercurrent disease, while donors were culled at preclinical stages or left to develop clinical disease.
We showed that blood can become infectious at early stages of sheep TSE infections and that the PrPd immunohistochemical phenotype of experimental sheep BSE was maintained after blood transfusion. Variability was observed within and between scrapie donors and recipients which might be attributable to the presence of more than one strain within the original source. Thus, the experimental sheep BSE model suggests that a change in pathological phenotype of vCJD would not be expected as a result of exposure through blood transfusion. We conclude that prion diseases of sheep can be of use to validate human blood diagnostic tests.
AD Sílvia Sisó (s.siso@vla.defra.gsi.gov.uk), Lorenzo González (l.gonzalez@vla.defra.gsi.gov.uk), Stuart Martin, Martin J. Jeffrey (m.jeffrey@vla.maff.gov.uk), Veterinary Laboratories Agency - Lasswade, Pentlands Science Park, Bush Loan, Midlothian EH26 0PZ, UK; E. Fiona Houston (fiona.houston@bbsrc.ac.uk), Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berkshire RG20 7NN, UK; Nora Hunter (nora.hunter@bbsrc.ac.uk), Institute for Animal Health, BBSRC and MRC, Neuropathogenesis Unit, Ogston Building, West Mains Road, Edinburgh EH9 3JF, Scotland, UK
SP englisch
PO UK