NR AWTS
AU Lacroux,C.; Corbiere,F.; Tabouret,G.; Lugan,S.; Costes,P.; Mathey,J.; Delmas,J.M.; Weisbecker,J.L.; Foucras,G.; Cassard,H.; Elsen,J.M.; Schelcher,F.; Andreoletti,O.
TI Dynamics and genetics of PrPsc placental accumulation in sheep
QU Journal of General Virology 2007 Mar; 88(3): 1056-61
PT journal article; research support, non-u.s. gov't
AB Placentae from scrapie-affected ewes are an important source of contamination. This study confirmed that scrapie-incubating ewes bearing susceptible genotypes could produce both abnormal prion protein (PrPsc)-positive and -negative placentae, depending only on the PRP genotype of the fetus. The results also provided evidence indicating that scrapie-incubating ARR/VRQ ewes may be unable to accumulate prions in the placenta, whatever the genotype of their progeny. Multinucleated trophoblast cells appeared to play a key role in placental PrPsc accumulation. PrPsc accumulation began in syncytiotrophoblasts before disseminating to uninucleated trophoblasts. As these result from trophoblast/uterine epithelial cell fusion, syncytiotrophoblast cells expressed maternal and fetal PrPc, whilst uninucleated trophoblast cells only expressed fetal PrPc. In ARR/VRQ scrapie-infected ewes, expression of the ARR allele by syncytiotrophoblasts appeared to prevent initiation of PrPsc placental deposition. The absence of prions in affected ARR/VRQ sheep placentae reinforces strongly the interest in ARR selection for scrapie control.
MH Alleles; Animals; Female; Fetus; Genotype; Immunohistochemistry; Placenta/*chemistry/pathology; PrPsc Proteins/*analysis; Pregnancy; Pregnancy Complications/metabolism/pathology/*veterinary; Scrapie/*metabolism/pathology; Sheep; Trophoblasts/chemistry
AD UMR INRA ENVT 1225, Interactions Hote Agent Pathogene, Ecole Nationale Veterinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
SP englisch
PO England