NR AWUA
AU Bencsik,A.A.; Philippe,S.; Debeer,S.; Crozet,C.A.; Calavas,D.; Baron,T.G.M.
TI Scrapie strain transmission studies in ovine PrP transgenic mice reveal dissimilar susceptibility
QU Histochemistry and Cell Biology 2007 May; 127(5): 531-9
PT journal article; research support, non-u.s. gov't
AB The Tg(OvPrP4) mouse line, expressing the sheep prion protein, is a sensitive model crucial for the identification of the bovine spongiform encephalopathy agent possibly present in natural sheep spongiform encephalopathies. It was also previously demonstrated as susceptible to infection with natural scrapie isolates from sheep harbouring various genotypes. The performance of this new transgenic mouse line in scrapie strain characterization was further assessed by intracranial inoculation of five groups of Tg(OvPrP4) mice with brain homogenate of the wild type mouse-adapted scrapie strains, C506M3, 22A, 79A, 87V, or Chandler. The Tg(OvPrP4) mice were susceptible to the scrapie agent transmitted using mouse-adapted scrapie strains but not equivalently. Strains 87V and Chandler were most readily transmissible followed by 79A and C506M3. Strain 22A was the least transmissible. Clinical signs, survival data, spongiosis, and PrPsc distribution were also reported. These various data demonstrate the possibility of distinguishing between scrapie strains. Our findings are discussed with regard to agent strain and host factors and already demonstrate the dissimilar susceptibilities of Tg(OvPrP4) mice to the different murine strains studied, thus, reinforcing their potential use in strain typing studies.
MH Animals; Brain/metabolism/pathology; Disease Susceptibility/metabolism/pathology; Female; Mice; Mice, Knockout; Mice, Transgenic; PrPsc Proteins/genetics/*metabolism; Scrapie/genetics/*metabolism/transmission; Sheep; Survival Analysis
AD Anna Bencsik (a.bencsik@lyon.afssa.fr), Sabine Debeer (s.debeer@freesurf.fr), Carole Crozet, Thierry Baron (t.baron@lyon.afssa.fr), ATNC unit, Agence Francaise de Securite Sanitaire des Aliments (AFSSA), 31 avenue Tony Garnier, 69364, Lyon Cedex 07, France; Sandrine Philippe (sandrine.philippe@la-ser.com), Didier Calavas (d.calavas@lyon.afssa.fr), Epidemiology Unit, Agence Française de Sécurité Sanitaire des Aliments (AFSSA), 31 avenue Tony Garnier, 69364 Lyon Cedex 07, France; Carole Crozet, Laboratoire de Biologie Moléculaire et Cellulaire, Agence Française de Sécurité Sanitaire des Aliments (AFSSA), 31 avenue Tony Garnier, 69364 Lyon Cedex 07, France (Present Address: UPR1142, Institut de génétique humaine, 34396 Montpellier, France); Sandrine Philippe (sandrine.philippe@la-ser.com), present address: LA-SER, Paris Biotech, 24, rue du Faubourg, Saint Jacques, 75014 Paris, France
SP englisch
PO Deutschland