NR AWWJ
AU Sawiris,G.P.; Becker,K.G.; Elliott,E.J.; Moulden,R.; Rohwer,R.G.
TI Molecular analysis of bovine spongiform encephalopathy infection by cDNA arrays
QU Journal of General Virology 2007 Apr; 88(4): 1356-62
PT journal article; research support, n.i.h., extramural; research support, u.s. gov't, non-p.h.s.
AB Here, the first cDNA array analysis of differential gene expression in bovine spongiform encephalopathy (BSE) is reported, using a spotted cDNA array platform representing nearly 17 000 mouse genes. Array analysis identified 296 gene candidates for differential expression in brain tissue from VM mice in late-stage infection with the 301V strain of BSE, compared with brain tissue from normal, age-matched VM mice. Real-time PCR confirmed differential expression of 25 of 31 genes analysed. Some of the genes identified by array analysis as being expressed differentially are associated with ubiquitin/proteasome function, lysosomal function, molecular chaperoning of protein folding or apoptosis. Other genes are involved in calcium ion binding/homeostasis, zinc ion binding/homeostasis or regulation of transcription. Principal-component analysis shows that the global gene-expression profiles of the BSE-infected samples have gene-expression signatures that are markedly different from, and completely non-overlapping with, those obtained from the normal controls.
MH Animals; Brain/pathology; Cattle; Encephalopathy, Bovine Spongiform/*genetics/pathology; *Gene Expression; *Gene Expression Profiling; Mice; Oligonucleotide Array Sequence Analysis; RNA, Messenger/analysis; Reverse Transcriptase Polymerase Chain Reaction
AD Research Service, VA Maryland Healthcare System, Baltimore, MD, USA
SP englisch
PO England