NR AWWV
AU Marcos-Carcavilla,A.; Calvo,J.H.; Gonzalez,C.; Moazami-Goudarzi,K.; Laurent,P.; Bertaud,M.; Hayes,H.; Beattie,A.E.; Serrano,C.; Lyahyai,J.; Martin-Burriel,I.; Alves,E.; Zaragoza,P.; Badiola,J.J.; Serrano,M.
TI IL-1 family members as candidate genes modulating scrapie susceptibility in sheep: localization, partial characterization, and expression.
QU Mammalian Genome 2007 Jan; 18(1): 53-63
PT journal article
AB Scrapie (SC) is a transmissible spongiform encephalopathy (TSE) in sheep and goats. Susceptibility to this neurodegenerative disease is controlled mainly by point mutations at the PRNP locus. Other genes, apart from PRNP, have been reported to modulate resistance/susceptibility to SC. On the basis of several studies on Alzheimer's disease and different TSE models, and of requirement for correct homeostasis of cytokines in brain, IL1B and IL1RN were chosen as putative positional and functional candidate genes that might be involved in the polygenic variance mentioned above. In the present work, ovine IL1B and IL1RN genes were partially isolated and characterized, including promoter and other regulatory regions. In addition, several sequence polymorphisms were identified. Furthermore, their cytogenetic positions on sheep chromosomes were determined by FISH and confirmed by linkage analysis, localizing both genes in OAR3p22, a region previously described as carrying a QTL for SC incubation period in sheep. Finally, expression analyses were carried out in eight naturally SC-infected and five uninfected sheep with the same genotype for PRNP (ARQ/ARQ). This comparison was performed using real-time RT-PCR in samples of spleen and cerebellum. Results showed differences in the expression of both cytokines in cerebellum (p < 0.05) but not in spleen (p > 0.05).
IN Marcos-Carcavilla et al. konnten durch Fluoreszenz-Hybridisierung in situ im Schaf die Gene für die Cytokine Interleukin 1b und Interleukin 1-1Ra in einer OAR3p22 genannten Region auf dem Schaf-Chromosom 3 lokalisieren, deren Einfluss auf die TSE-Empfänglichkeit für das Hausmaus-Modell bereits gezeigt worden sein soll.
MH Animals; Base Sequence; Cerebellum/immunology; Chromosome Mapping; DNA Primers/genetics; DNA, Complementary/genetics; Gene Expression; Genetic Predisposition to Disease; Interleukin 1 Receptor Antagonist Protein/genetics; Interleukin-1/*genetics; Interleukin-1ß/genetics; Molecular Sequence Data; Polymorphism, Genetic; Prions/genetics; RNA, Messenger/genetics/metabolism; Scrapie/*genetics/*immunology; Sheep/*genetics/*immunology
AD Ane Marcos-Carcavilla (amarcos@inia.es), Carmen González, Estefânia Alves, Magdalena Serrano, Departamento de Mejora Genética Animal, INIA, Ctra La Coruna Km 7.5, 28040, Madrid, Spain; Jorge H. Calvo, Unidad de Tecnología en Producción Animal, Avda. Montanana 930, CITA, 50059, Zaragoza, Spain; Katayoun Moazami-Goudarzi, Pascal Laurent, Maud Bertaud, Hélène Hayes, Laboratoire de Génétique biochimique et de Cytogénétique, Département de Génétique Animale, INRA, Centre de Recherche de Jouy, 78352, Jouy-en-Josas, Cedex, France; Anne E. Beattie, AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, New Zealand; Carmen Serrano, Jaber Lyahyai, Inmaculada Martín-Burriel, Pilar Zaragoza, Laboratorio de Genética Bioquímica, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Server 177, 50013, Zaragoza, Spain; Juan J. Badiola, Centro Nacional de Referencia de EETs, Universidad de Zaragoza, Miguel Server 177, 50013, Zaragoza, Spain
SP englisch
PO USA