NR AWXO
AU Vranac,T.; Hartman,K.P.; Popovic,M.; Venturini,A.; Zerovnik,E.; Curin Serbec,V.
TI A single prion protein peptide can elicit a panel of isoform specific monoclonal antibodies
QU Peptides 2006 Nov; 27(11): 2695-705
PT journal article; research support, non-u.s. gov't
AB The main step in the pathogenesis of transmissible spongiform encephalopathies (TSE) is the conformational change of the normal cellular prion protein (PrPc) into the abnormal isoform, named prion (PrPsc). Since PrP is a highly conserved protein, the production of monoclonal antibodies (mAbs) of high specificity and affinity to PrP is a difficult task. In the present study we show that it is possible to overcome the unresponsiveness of the immune system by immunizing wild-type BALB/c mice with a 13 amino acid PrP peptide from the C-terminal part of PrP, bound to the keyhole limpet hemocyanin (KLH). Immunization induced predominantly anti-PrPsc humoral immune response. Furthermore, we were able to obtain a panel of mAbs of IgG class specific for different non-self-conformations of PrP, with anti-PrPsc-specific mAbs being the most abundant.
MH Amino Acid Sequence; Animals; Antibodies, Monoclonal/*biosynthesis; Antibody Specificity; Blotting, Western; Brain/immunology; Circular Dichroism; Humans; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Peptides/administration & dosage/*genetics/*immunology; Prions/*genetics/*immunology
AD Blood Transfusion Centre of Slovenia, Slajmerjeva 6, SI-1000 Ljubljana, Slovenia.
SP englisch
PO USA