NR AXAF
AU Brun,A.; Gutierrez-Adan,A.; Castilla,J.; Pintado,B.; Diaz-San Segundo,F.; Cano,M.J.; Alamillo,E.; Espinosa,J.C.; Torres,J.M.
TI Reduced susceptibility to bovine spongiform encephalopathy prions in transgenic mice expressing a bovine PrP with five octapeptide repeats
QU Journal of General Virology 2007 Jun; 88(6): 1842-9
PT journal article; research support, non-u.s. gov't
AB In this work, transgenic (Tg) mice were generated expressing a bovine prion protein containing five octarepeats (BoPrP(5OR)-Tg). After intracerebral inoculation of bovine spongiform encephalopathy (BSE) inoculum, these mice suffered a BSE-like neuropathology but survived longer compared with homologous Tg mice expressing similar levels of a six octarepeat BoPrP protein (BoPrP(6OR)-Tg). De novo-generated five octarepeat (5OR) PrPsc showed no biochemical differences from 6OR-PrPsc, and the proteinase K-resistant core (PrPres) was biochemically indistinguishable from the 6OR counterpart. Lower susceptibility to BSE is suggested for BoPrP(5OR)-Tg mice, as they were not as efficient at replicating BSE prions from the same natural source inoculum as BoPrP(6OR)-Tg mice expressing similar PrPc levels. These results raise the possibility of selecting cattle breeds bearing the 5OR Prnp allele that are less susceptible to prion infection.
IN Mit einem BSE-Inokulum inokulierten Brun et al. intrazerebral transgene Mäuse, deren Rinder-Prionproteine entweder 5 oder 6 Octapeptidsequenzen enthielten. Bei den Mäusen mit den um ein Octapeptid verkürzten Prionproteinen dauerte die Inkubationszeit länger, aber biochemisch konnten die Autoren die PrPsc mit 5 oder 6 Octapeptidregionen nicht unterscheiden.
MH Animals; Brain/pathology; Cattle; Disease Models, Animal; Disease Susceptibility; Encephalopathy, Bovine Spongiform/*genetics/pathology; Immunohistochemistry; Mice; Mice, Transgenic; Prions/*genetics; Repetitive Sequences, Nucleic Acid/*genetics
AD Centro de Investigacion en Sanidad Animal, INIA, Valdeolmos, 28130 Madrid, Spain.
SP englisch
PO England