NR AXBB
AU Sakudo,A.; Nakamura,I.; Ikuta,K.; Onodera,T.
TI Recent developments in prion disease research: diagnostic tools and in vitro cell culture models.
QU Journal of Veterinary Medical Science 2007 Apr; 69(4): 329-37
PT journal article; research support, non-u.s. gov't; review
AB After prion infection, an abnormal isoform of prion protein (PrPsc) converts the cellular isoform of prion protein (PrPc) into PrPsc. PrPc-to-PrPsc conversion leads to PrPsc accumulation and PrPc deficiency, contributing etiologically to induction of prion diseases. Presently, most of the diagnostic methods for prion diseases are dependent on PrPsc detection. Highly sensitive/accurate specific detection of PrPsc in many different samples is a prerequisite for attempts to develop reliable detection methods. Towards this goal, several methods have recently been developed to facilitate sensitive and precise detection of PrPsc, namely, protein misfolding cyclic amplification, conformation-dependent immunoassay, dissociation-enhanced lanthanide fluorescent immunoassay, capillary gel electrophoresis, fluorescence correlation spectroscopy, flow microbead immunoassay, etc. Additionally, functionally relevant prion-susceptible cell culture models that recognize the complexity of the mechanisms of prion infection have also been pursued, not only in relation to diagnosis, but also in relation to prion biology. Prion protein (PrP) gene-deficient neuronal cell lines that can clearly elucidate PrPc functions would contribute to understanding of the prion infection mechanism. In this review, we describe the trend in recent development of diagnostic methods and cell culture models for prion diseases and their potential applications in prion biology.
ZR 143
MH Animals; Biological Assay/veterinary; Blotting, Western/veterinary; Cell Culture Techniques/veterinary; Enzyme-Linked Immunosorbent Assay/veterinary; Immunohistochemistry/veterinary; PrPsc Proteins/*isolation & purification; Prion Diseases/*diagnosis/*veterinary
AD Department of Molecular Immunology, School of Agricultural and Life Sciences, The University of Tokyo, Japan.
SP englisch
PO Japan