NR AXFM
AU Jin,J.K.; Na,Y.J.; Song,J.H.; Joo,H.G.; Kim,S.; Kim,J.I.; Choi,E.K.; Carp,R.I.; Kim,Y.S.; Shin,T.
TI Galectin-3 expression is correlated with abnormal prion protein accumulation in murine scrapie
QU Neuroscience Letters 2007 Jun 13; 420(2): 138-43
PT journal article; research support, non-u.s. gov't
AB To investigate the involvement of galectin-3 in the process of neurodegeneration in prion diseases, the expression and cellular localization of galectin-3 in the brain were studied in scrapie, a mouse model of prion disease. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analyses showed that the expression of galectin-3 protein and mRNA was induced in scrapie-affected brains, particularly at the time when the abnormal prion protein PrPsc began to accumulate in the brains. Immunohistochemically, immunostaining for galectin-3 was found mainly in B4-isolectin-positive cells (presumably activated microglia/macrophages), but not in astrocytes. Galectin-3 immunoreactivity was localized mainly in areas of PrPsc accumulation and neuronal death in scrapie-infected brains. These findings suggest that the expression of galectin-3 by activated microglia/macrophages in prion disease correlates with abnormal prion protein accumulation.
MH Animals; Biological Markers/analysis/metabolism; Brain/*metabolism/pathology/physiopathology; Galectin 3/genetics/*metabolism; Glial Fibrillary Acidic Protein/metabolism; Gliosis/metabolism/physiopathology; Macrophages/metabolism; Mice; Mice, Inbred C57BL; Microglia/metabolism; Nerve Degeneration/etiology/metabolism/physiopathology; Neurons/metabolism/pathology; Plant Lectins; PrPsc Proteins/*metabolism; RNA, Messenger/metabolism; Scrapie/*metabolism/physiopathology; Up-Regulation/physiology
AD Ilsong Institute of Life Science, Hallym University Medical Center, Anyang, Kyonggi-do 431-060, South Korea.
SP englisch
PO Irland