NR AXGA
AU Takenouchi,T.; Iwamaru,Y.; Imamura,M.; Kato,N.; Sugama,S.; Fujita,M.; Hashimoto,M.; Sato,M.; Okada,H.; Yokoyama,T.; Mohri,S.; Kitani,H.
TI Prion infection correlates with hypersensitivity of P2X7 nucleotide receptor in a mouse microglial cell line
QU FEBS Letters 2007 Jun 26; 581(16): 3019-26
PT journal article; research support, non-u.s. gov't
AB We recently established mouse microglial cells persistently infected with mouse-adapted scrapie ME7 (ScMG20/ME7) for in vitro study of prion pathogenesis. Here, we found that ScMG20/ME7 cells were hypersensitive to P2X7 receptor agonists, as demonstrated by sustained Ca(2+) influx, membrane pore formation, cell death, and interleukin-1beta release. P2X7 mRNA expression was upregulated in these cells, and also in scrapie-infected mice brains. Treatment with pentosan polysulfate eliminated the infectivity and disease-related forms of prion protein from ScMG20/ME7 cell cultures, however, hypersensitivity of P2X7 receptors remained. These results suggest that prion infections may strongly affect the P2X7 receptor system in mouse microglial cells.
MH Adenosine Triphosphate/analogs & derivatives/*pharmacology; Animals; Calcium Signaling/drug effects; Cell Line; Dose-Response Relationship, Drug; Mice; Mice, Inbred Strains; Microglia/drug effects/*pathology; Pentosan Sulfuric Polyester/pharmacology; Prions/*pathogenicity; Receptors, Purinergic P2/*agonists/metabolism/physiology; Scrapie/*pathology
AD Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Ohwashi 1-2, Tsukuba, Ibaraki 305-8634, Japan.
SP englisch
PO Niederlande