NR AXGT
AU Rodriguez,A.; Ferrer,I.
TI Expression of transcription factors CREB and c-Fos in the brains of terminal Creutzfeldt-Jakob disease cases
QU Neuroscience Letters 2007 Jun 21; 421(1): 10-5
PT journal article; research support, non-u.s. gov't
AB Expression levels and localization of transcription factors cAMP response element binding protein (CREB(1) and CREB(2)) and c-Fos, as well as levels of up-stream mitogen-activated protein kinases/extracellular signal-regulated kinases (ERK-1 and ERK-2) and p38 kinase, were examined in the brains (frontal cortex) of eleven cases with Creutzfeldt-Jakob disease (CJD) and five age-matched controls. Preserved expression levels of ERK-1-P, ERK-2-P and p38-P were observed in CJD. However, significantly reduced levels, as revealed by gel electrophoresis and Western blotting, and reduced numbers of immunoreactive nuclei, as seen by immunohistochemistry, to CREB, CREB-P, c-Fos and c-Fos-P were found in CJD when compared with controls. These observations point to exhausted CREB and c-Fos brain responses, in spite of preserved up-stream signaling kinases, thus favoring cell death in terminal stages of CJD.
MH Aged; Aged, 80 and over; CREB-Binding Protein/*metabolism; Case-Control Studies; Creutzfeldt-Jakob Syndrome/genetics/metabolism/*pathology; Female; Gene Expression/physiology; Gene Expression Regulation/*physiology; Humans; Immunohistochemistry; Male; Middle Aged; Mitogen-Activated Protein Kinases/metabolism; Postmortem Changes; Proto-Oncogene Proteins c-fos/*metabolism
AD Institut de Neuropatologia, Servei Anatomia Patologica, IDIBELL-Hospital Universitari de Bellvitge, Universitat de Barcelona, Feixa Llarga sn, 08907 Hospitalet de Llobregat, Spain.
SP englisch
PO Irland