NR AXHN
AU Abdel-Haq,H.; Lu,M.; Liu,Q.; Puopolo,M.; Cardone,F.
TI Effectiveness of Phthaloacynine Tetrasulfonate Against Rodent-Adapted Human TSE Strains
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.65
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB It became increasingly urgent the need of an effective therapy for the human transmissible spongiform encephalopathy (TSE) diseases especially after the evidence of the potential inter-human transmissibility of the variant CJD by blood. None of the many tested compounds for an anti-TSE activity had revealed a therapeutic effectiveness in the advanced stage of the pathology. However a cyclic tetrapyrrole compound, phthalocyanine tetrasulfonate (PcTS) has recently demonstrated to be effective against 2 different rodent-adapted scrapie strains 263K and RML, suggesting that it might be potentially useful also against human TSE strains. We have tested the effect of PcTS on mouse-adapted human TSE strains isolated from variant CJD (mvCJD strain) and Gerstmann Sträussler syndrome (KFu strain), and on rodentadapted scrapie strains (mouse strain 139A and hamster strain 263K). Groups of 9-13 animals were intraperitoneally treated with 10 mg/Kg PcTS 3 times/week for 4 weeks starting immediately after the intraperitoneal infection with 10% brain homogenate of mvCJD, KFu and 139A in C57BL mice and 263K in golden Syrian hamsters. PcTS treatment prolonged significantly the median survival time with respect to controls in mice inoculated with KFu (351 vs 401, P<=0.003), 139A (209 vs 235, P<=0.0001) and mvCJD (271 vs 310, P<=0.03). But, it did not significantly prolong the median survival time (113 vs 140, P=0.2) in hamsters inoculated with 263K scrapie strain. PcTS showed its highest effectiveness against the mouse-adapted human strains KFu and vCJD. Since data in the literature and ours, demonstrated that the maximum anti-TSE activity of PcTS occurs at time of infection, we conclude that PcTS could be proposed as a potential candidate for an early treatment of human TSE diseases when a reliable preclinical test will be available.
AD H. Abdel-Haq, M. Lu, Q. Liu, M. Puopolo, F. Cardone, Istituto Superiore di Sanità, Italy
SP englisch
PO Schottland