NR AXHP
AU Adjou,K.T.; Humeau,G.; Messiaens,S.; El Hachimi,K.H.; Ouidja,M.O.; Couquet,C.; Deslys,J.P.; Eloit,M.; Brugere-Picoux,J.
TI Characterization of a Highly Pathogenic Isolate of Sheep Scrapie in Transgenic Mice Overexpressing Murine PrP
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Natural and Experimental Strains P02.25
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB The characteristics of scrapie strain circulating in small ruminants has not been extensively investigated. The aim of this study was the characterization of the clinical, pathological and biochemical aspects of a highly pathogenic isolate of natural scrapie in sheep called "TAC". Transgenic mice overexpressing normal murine PrP (Tga20) have been inoculated intracerebrally with this isolate. This study has been performed in collaboration with the French scrapie surveillance network. This scrapie strain was first isolated from a lamb which has been probably infected by maternal transmission. This lamb developed fastly the disease after only 6 months of incubation (2 to 3 years normally). A histological study and a molecular characterization of the strain were performed on the infected mice brain and a strain typing was established. The inoculated mice exhibited a very short incubation period (105 ± 18 days) compared to other strains (i.e. 599 ± 120 days for O69 scrapie strain) clinical signs were not obvious, and mice died suddenly. The lesion profile identified is close to that of other scrapie strains profile and clearly different from the BSE one. The PrPsc was detected in several areas of the mice brains as diffused and granular aggregates and more specifically into the thalamus, the cerebellum and the choroid plexus. The GFAP detection revealed a strong astrocytosis. Furthermore, hyperchromatic neurones were observed in "TAC" scrapie infected mice indicating a severe neurodegeneration. Finally, the PrPsc molecular profiles have confirmed that the "TAC" isolate is a classical scrapie strain.
AD K. Adjou, G. Humeau, M.O. Ouidja, J. Brugere-Picoux, Ecole Nationale Veterinaire D'Alfort, Laboratoire de Pathologie du Bétail, France; S. Messiaens, Ecole Nationale Veterianire D'Alfort, UMR 1161 Virologie, France; K.H. El Hachimi, Hopital La Salpetriere, France; C. Couquet, Laboratoire Vetrinaire Departemental, France; J.-P. Deslys, CEA, Serpia, France; M. Eloit, Ecole Nationale Veterinaire D'Alfort, Virologie, France
SP englisch
PO Schottland