NR AXHS
AU Akimov,S.; Yakovleva,O.; McKenzie,C.; Vasilyeva,I.; Cervenakova,L.
TI Can Retinal Pigment Epithelial (RPE) Cells Be Infected with TSE Agents?
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.42
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
Retinal pigment epithelial (RPE) cells are in close contact with neuronal retinal cells, and are responsible for phagocytosis of 'outer fragments' of photoreceptors. Infectivity in the retina has repeatedly been demonstrated in animals with experimental and natural scrapie, and PrPTSE has been found in the plexiform layers in two cases of sporadic CJD (sCJD) and two cases of variant CJD (vCJD). We aimed to investigate whether RPE cells can be infected with various transmissible spongiform encephalopathy (TSE) agents in cell culture. Adult RPE (ARPE-19) cells were exposed to the mouse-adapted GSS strain (Fukuoka-1), mouse-adapted vCJD (mo-vCJD), vCJD, sCJD and BSE for 96 hours and carried through 15 passages. The presence of PrPc and PrPTSE was demonstrated using 6D11 antibodies by cell blot or western
blot of the cultured cells 96 hours following inoculation, and after 5, 10 and 15 passages. We showed that ARPE-19 cells express PrPc through all passages; but did not find PrPTSE in cell lysates in any passage following inoculation with Fukuoka-1. A small amount of PrPTSE was detected in cells exposed to mo-vCJD at 96 hours after inoculation by cell and western blot. Results of tests to elucidate the susceptibility of ARPE-19 cells to BSE, vCJD and sCJD(not yet completed) will be presented and
discussed.
AD S. Akimov, O. Yakovleva, C. McKenzie, I. Vasilyeva, L. Cervenakova, J.H. Holland Laboratory, USA
SP englisch
PO Schottland