NR AXIE
AU Arsac,J.N.; Biacabe,A.G.; Nicollo,J.; Bencsik,A.; Baron,T.G.M.
TI Biochemical Identification of Bovine Spongiform Encephalopathies in Cattle
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.168
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB Important changes have occurred in the post-mortem diagnosis of Bovine Spongiform Encephalopathy (BSE) in recent years. The methods used for confirmatory diagnosis of BSE should follow the OIE guidelines and continually need to be reassessed. We have evaluated a commercially available Western blot method (TeSeE(R) Wb) as a potential means of confirming BSE. This method was (i) highly sensitive, compared to previously used biochemical confirmatory methods and (ii) more sensitive than 2 routinely used highly sensitive rapid tests (TeSeE(R) Elisa, HerdCheck(R) BSE). We show that this high sensitivity is mainly due to the antibody used (Sha31). Interestingly, TeSeE(R) Wb was also able to diagnose the two currently recognised deviant BSE phenotypes (H-Type and L-Type or BASE). The initially described molecular features of these atypical forms of BSE were also readily recognised, although sensitivity of the method may be differently affected by the chosen Ab compared to typical BSE. This method is thus of potential interest for future evaluations of BSE confirmatory methods.
AD J.-N. Arsac, Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Unité ATNC, France; A.-G. Biacabe, J. Nicollo, A. Bencsik, T. Baron, AFSSA (Agence Française de Sécurité Sanitaire des Aliments), France
SP englisch
PO Schottland