NR AXII
AU Avrahami-Tzfati,D.; Dayan-Amouyal,Y.; Tal,S.; Mincberg,M.; Gabizon,R.
TI Infection of Minute Virus of Mice (MVM) Increases the Binding and Internalization of Exogenous PrPsc into A9 Fibroblasts
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.171
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB The possible effect of intercurrent viral infections on prion disease pathogenesis is poorly understood. In this study, we show that fibroblasts infected with Minute Virus of Mice (MVM) bind and internalize significantly more exogenous PrPsc as compared to naive cells. This was further confirmed by the observation of enhanced localization of the internalized prion protein to the lysosomes in MVM-infected cells. In addition, the internalized PrPsc distributed differently in the MVM-infected cells, as demonstrated by the shift in migration of PrPsc through floatation gradients toward the lighter fractions where the Raft residents ganglioside GM1 and caveolin 1 are present. Interestingly, the MVM-infected fibroblasts demonstrated a considerable increase in both Raft markers, suggesting that viral infection can significantly alter the cell lipid composition. Such an alteration may facilitate the incorporation of PrPsc molecules into the Rafts. Since the conversion of PrPc to PrPsc is believed to occur in such membrane micro domains, changes in cell lipid composition due to viral infection may affect the first steps of prion disease initiation.
AD D. Avrahami-Tzfati, Y. Dayan-Amouyal, R. Gabizon, Hadassah Hebrew University Medical Center, Israel; S. Tal, M. Mincberg, Ben Gurion University of the Negev, Israel
SP englisch
PO Schottland
EA pdf-Datei und Poster (Postertitel: Viral induced alterations of membrane lipids affect the incorporation of PrPrSc into cells)