NR AXIV
AU Barr,J.; Ironside,J.W.; Watson,M.; Harris,N.; Fraser,J.R.; Barron,R.
TI A Proteomics Approach to Establishing New Surrogate Markers for the Diagnosis of TSE Disease
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.165
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
The prion protein as a single marker protein is the basis of all commercially available diagnostic tests for the detection of TSEs in animals and humans. Whilst this has been successful in post mortem testing it is difficult to detect in blood thus restricting use as an ante-mortem diagnostic. There are also doubts, with the emergence of atypical strains of scrapie and BSE, that it may lack the sensitivity required for a definitive diagnostic marker of TSE disease.
Considering the above and our interest in understanding the processes involved in the neurodegenerative changes taking place within the CNS of affected animals, we have used a proteomics approach using surface enhanced desorption/ionisation(SELDI) mass spectrography to establish new surrogate markers of TSE disease. Our approach to finding new surrogate markers uses and SELDI-MS-TOF technology in brain samples from a well characterised murine scrapie model to establish a panel of markers for scrapie diagnosis. The murine model used in this experiment (intracerebrally injected with ME7scrapie isolate) displays severe pathology in the hippocampus where it was thought that the highest number of disease specific markers would be found. Many potential biomarker profiles were detected by this method, some in the early stages of disease before clinical signs were obvious. The profiles can be used collectively as a panel of markers without formal identification however, by identifying individual proteins we can also establish potential single markers and their role in TSE pathogenesis. We have purified and identified individual proteins using mass spectrometry including SELDI, and western blotting techniques. The identified proteins were then localised in brain sections using immunocytochemical techniques.
In collaboration with the German Primate Centre, we have also examined simian CSF from TSE infected animals using differential protein expression profiling, for protein markers of disease.
AD J. Barr, J. Fraser, R. Barron, Roslin Institute, Neuropathogenesis Unit, UK; J.W. Ironside, University of Edinburgh, Western General Hospital, CJD Surveillance Unit, UK; M. Watson, Institute for Animal Health, Bioinformatics, UK; N. Harris, Ciphergen Biosystems Ltd, UK
SP englisch
PO Schottland
EA pdf-Datei und Poster (Posterautoren um D. Motzkus ergänzt)