NR AXJQ
AU Borthwick,E.B.; Brenn,A.; Groschup,M.H.; Archibald,A.L.; Williams,J.L.
TI There is Differential Gene Expression in Cattle White Blood Cells During Preclinical BSE Infection
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.150
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB Differential gene expression within peripheral tissues of BSE infected cattle could provide the means of identification for early BSE infection. The oral route of infection by the infected agent (PrPres) passes from the gastrointestinal system to the CNS via the lymporeticular system. Little is known of the effect on gene expression of the host organs during this early infection period; although there is some evidence of decreased levels of erythroid differentiation-related factor (EDRF) in the spleen and erythroid cells in the blood of TSE infected mice. Therefore there is a possibility of using differential gene expression in peripheral tissues as an early diagnostic test for BSE infection. In this project we are investigating the differential gene expression between normal and BSE infected cattle using RNA isolated from white blood cells. Blood samples were collected from a BSE challenge set up at Greifswald, Germany using Simmental cattle. Samples were collected from 7 months post infection and subsequently every 2 months during the BSE incubation. Initially samples were fractionated into PBMs (peripheral blood monocytes) and at later time points individual white blood cells were separated into B cells, T cells and macrophages. RNA isolated from the blood samples was used to probe the Affymetrix Bovine Genome Array. Analysis of the microarray experiments produced gene lists of significantly differentially expressed genes. There are many genes which have varying expression patterns throughout the time course however there are some genes which are consistently down regulated across most cell types for example TRYP8(pancreatic anionic trypsinogen). QPCR studies have confirmed the TRYP8 expression patterns and further QPCR experiments are being performed to follow up related proteins to investigate the significance of this finding. Other genes found to be significantly up regulated include members of the chemokine family, both ligands and receptors and immune response pathways. Again these have been confirmed by QPCR and are being further investigated using related proteins by QPCR. It is hoped that these findings are due to the specific disease pathology of BSE during preclinical infection and if this is the case then the differential gene expression could be used as an early signal for the incubation of BSE in cattle.
AD E.B. Borthwick, A.L. Archibald, J.L. Williams, Roslin Institute, Genetics and Genomics, UK; A. Brenn, M.H. Groshup, Federal Reserach Institute for Animal Health, Friedrich-Löffler-Institut, Germany
SP englisch
PO Schottland
EA pdf-Datei und Poster (Posterautoren ergänzt um R. Talbot und S. Hawkins)