NR AXKB
AU Buschmann,A.; Groschup,M.H.
TI Limited Efficacy of 3-Methyl-4-Chlorophenol (Neopredisan 135-1) for the BSE Prion Decontamination in Homogenized Tissue
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.121
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
The potential application of Neopredisan 135-1 for the decontamination of TSEcontaminated medical devices has been analysed using the 263K Hamster model recently (1). In order to examine the potency of Neopredisan 135-1 for the inactivation of the BSE agent, we performed similar experiments to those described in the 263Kmodel with brain material from BSE infected cattle. Briefly, a dilution series of a 10% brain homogenate was incubated with Neopredisan 135-1 at different concentrations. After pH neutralisation the samples were either digested with proteinase K and analysed by immunoblot or inoculated into Tgbov XV mice overexpressing bovine PrPc. Both experiments indicate only a limited effectiveness of Neopredisan 135-1 for the inactivation of the BSE agent, as only minor differences in the results from samples with and without 1% or 2% Neopredisan 135-1 were observed. These results do not support a practical application of Neopredisan 135-1 for decontamination of animal facilities or slaughterhouses.
Reference: (1) C. Riemer, T. Bamme, S. W. F. Mok, and M. Baier, 2006:3-Methyl-4Chlorophenol for Prion Decontamination of Medical Devices. Infect Control Hosp Epidemiol. 27(7):778-80.
AD A. Buschmann, M.H. Groschup, Friedrich-Löffler-Institut, Institute for Novel and Emerging Infectious Diseases, Germany
SP englisch
PO Schottland