NR AXKE
AU Canello,T.; Engelstein,R.; Gasset,M.; Sklaviadis,T.; Xanthopoulos,K.; Langeveld,J.; Gabizon,R.
TI A Different Metabolic Isoform of PrPsc in Species Other then Mice
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Protein Misfolding P01.65
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB Nonglycosylated and monoglycosylated forms of mouse PrPsc, but not those from bovine, ovine or human brain samples, can be recognized by IPC2, a new aPrP mAb. Contrarily to its specificity for mouse PrPsc, IPC2 reacts willingly with all the corresponding recombinant PrPs. Deglycosylation of diverse brain samples did not confer to IPC2 affinity to non-reacting forms of PrP, suggesting its activity requires a free asparagine, as opposed to the aspartic acid resulting from deglycosylation of Nlinked sugars by PNGase. Immunoblotting of mice brain samples and recombinant rodent PrP in the absence of ßme significantly increased IPC2 reactivity, while reduction and alkylation of the disulfide bond almost abolish its activity, indicating the IPC2 epitope, in addition to a free asparagine, comprises an intact disulfide bond between residues C179 and C214. Consistent with this possibility, PEPscan studies fail to identify a linear epitope for IPC2 in the PrP sequence. We hypothesize that in species higher then mice, the lower bands of PrPsc may comprise mostly deglycosilated as opposed to non-glycosilated forms, resulting from the activity of cellular PNGases, thereby presenting aspartic acid at residue 180 instead of asparagine. Ongoing sequencing experiments will determine if indeed this is the case. e Whether this form of PrP editing affects prion propagation remains to be established.
AD T. Canello, R. Engelstein, R. Gabizon, Hadassah Hebrew University Medical Center, Laboratory of Experimental Neurology, Israel; M. Gasset, Instituto Quimica-Fisica "Rocasolano", Spain; T. Sklaviadis, K. Xanthopoulos, Aristotle University of Thessaloniki, Greece; J. Langeveld, Lelystad, Netherlands
SP englisch
PO Schottland
EA pdf-Datei und Poster (geringfügige Änderung in der Reihenfolge der Autoren)