NR AXKO
AU Cooper,J.K.; Ladhani,K.; Minor,P.
TI Direct Comparison of CJD Diagnostic Assays Using Identical Sample Sets
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.92
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB With the fourth reported case of vCJD transmission through blood transfusion there is an increased urgency to identify and implement a test for use in blood donor screening. Selection of an assay with sufficient sensitivity to detect blood-borne TSE infectivity is hampered by the lack of suitable samples such as blood from vCJD cases or from individuals known to have been exposed to vCJD i.e through transfusion. Alternative strategies for determining assay sensitivity are therefore needed. One approach is to take materials prepared from vCJD cases that have been characterised for PrPsc content and spike these materials into plasma at various concentrations. Several studies using such an approach have been undertaken by NIBSC and have highlighted the strengths and potential weaknesses of such an approach. Identical panel sets were used to directly compare the sensitivity and reproducibility of assays that are currently being developed for the diagnosis of vCJD. The results of these studies will be presented.
AD J. Cooper, K. Ladhani, NIBSC, CJD Resource Centre, UK; P. Minor, NIBSC, Virology, UK
SP englisch
PO Schottland