NR AXKX
AU Di Guardo,G.; Marruchella,G.; Ligios,C.; Baffoni,M.; Cancedda,G.M.; Macciocu,S.; Gioia,L.; Lalatta Costerbosa,G.; Chiocchetti,R.; Clavenzani,P.; De Grossi,L.; Agrimi,U.; Aguzzi,A.
TI Immunohistochemistry, Immunofluorescence and Confocal Laser Microscopy: An Insight into the Enteric Nervous System During Sheep Scrapie Infection
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.133
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
The enteric nervous system (ENS) is likely to play a role in the early pathogenesis of sheep scrapie, but little is known about the ENS cell types involved. We investigated the ileal myenteric plexi (MPs) and submucosal plexi (SMPs) of 4 natural and 4 oral experimental scrapie-affected ARQ/ARQ Sarda breed sheep, as well as of 12 healthy sheep carrying different PRNP genotypes. In all control animals, as well as in all scrapie-affected sheep, which were euthanized at the end stage of clinical disease, detailed laboratory investigations were carried out by means of immunohistochemistry (IHC), as well as by indirect immunofluorescence (IIF) and confocal laser microscopy (CLM) techniques.
All the above 8 scrapie-affected animals showed IHC evidence of PrPsc deposition within both MPs and SMPs, with IIF and CLM studies allowing us to identify enteroglial cells (EGCs) and, for the first time, also calbindin (CALB)-immunoreactive (IR) and neuronal nitric oxide synthase (nNOS)-IR neurons as the ENS cytotypes involved in PrPsc accumulation and plausibly, thereby, in the subsequent process of "neuroinvasion".
In conclusion, IHC, IIF and CLM proved to be in our hands three highly valuable and complementary laboratory techniques for investigating the pathogenesis of both natural and experimental sheep scrapie infection.
Footnote: This work was carried out with research grants (PRIN 2004, PRIN 2006) from the Italian Ministry for Education, University and Research (MIUR).
AD G. Di Guardo, G. Marruchella, M. Baffoni, L. Gioia, Faculty of Veterinary Medicine, University of Teramo, Dept Comparative Biomedical Sciences, Italy; C. Ligios, G.M. Cancedda, S. Macciocu, Istituto Zooprofilattico Sperimentale della Sardegna, Italy; G. Lalatta Costerbosa, R. Chiocchetti, P. Clavenzani, Faculty of Veterinary Medicine, University of Bologna, Dept Vet Morphophysiology and Animal Productions, Italy; L. De Grossi, Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana, Italy; U. Agrimi, Istituto Superiore di Sanità, Dept Food Safety and Veterinary Public Health, Italy; A. Aguzzi, University Hospital of Zürich, Institute of Neuropathology, Switzerland
SP englisch
PO Schottland