NR AXKZ
AU Dobson,C.; Navelkova,Z.; Buchbauerova,J.; Simmons,P.; Campos,S.; Garcia,D.; Cook,J.; Clarke,J.; Heath,N.; O'Brien,A.; Stumpf,R.
TI Evaluation of Plate Processing Instrumentation for Rapid TSE Assays
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Protein Misfolding P01.73
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
Automated ELISA plate handling has obvious and previously reported benefits with respect to laboratory throughput, bench space and labour intensity. This study assesses plate processing instrumentation with respect to pipetting capabilities and overall assay performance.
The Stratec 4PS instrument has been adapted by several companies for use as an automated analyser in the field of human blood testing and as such is, IVDD compliant, well established and ubiquitous. Enfer Scientific have adapted this machine for use as a plate processor for the ELISA part of the Enfer Version 3 rapid test for TSE. This Enfer adaptation is known as the en4lisa.
The Enfer TSE Version 3 test features coloured reagents which allows reagent addition verification to be performed on the en4lisa. This feature has been used in the study to assess pipetting precision throughout the individual steps of the assay. Additionally, assay performance has been assessed by means of, testing negative samples and repeatedly testing a blinded positive panel.
The study was performed under routine conditions in TSE testing laboratories in the Czech Republic, Ireland and Spain. The data show that the pipetting precision of the en4lisa is equivalent to, or better than, that normally achieved by manual users and the assay performance is consistent with the manual assay.
AD C. Dobson, J. Cook, N. Heath, Murex Biotech, UK; Z. Navelkova, J. Buchbauerova, Statni Venerinarni Ustav Olomuc, Czech Republic; P. Simmons, Enfer Testing, Ireland; S. Campos, D. Garcia, Laboratorio Regional de Sanidad y Producción Animal, Spain; J. Clarke, A. O'Brien, Enfer Scientific, Naas, Ireland; R. Stumpf, Abbott Diagnostics Division, USA
SP englisch
PO Schottland