NR AXLR
AU Ersdal,C.; Goodsir,C.M.; McGovern,G.; Simmons,M.M.; Jeffrey,M.
TI Association of PrPd with Cytopathological Changes of BSE Affected Cattle
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.108
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
Aims: In this study we have sought to determine the nature of sub-cellular changes in the dorsal motor nucleus of the vagus (DMNV) and the solitary tract nucleus (NTS) and their relationship to PrPd accumulation of bovine spongiform encephalopathy (BSE) affected cattle.
Methods: Nine naturally BSE infected cattle and three controls were perfusion fixed using mixed aldehydes. The DMNV and the NTS were identified and processed by standard methods to plastic. 65-nm thin sections were immunogold labeled with the R523.7 PrP antibody.
Results: Vacuolation was conspicuous and of two types: those that appeared as dissolution of the matrix of dendrites and those that appeared as expanding single walled structures that impinged and involved other processes. Areas of osmiophilic microvacuoles were found in several animals. In most of the infected animals there where restricted areas of small irregular profiles that had an increased electron density of the extracellular space. Membrane invaginations were found in dendrites. They often appeared as single structures, but could be found in pairs and have a branching structure. The membrane invaginations could be associated with surrounding areas of increased electron density in the extracellular space, membrane irregularities and other nearby invaginations. In axons and axon terminals, spiral membrane inclusions and synaptic vesicles arranged in clumps were seen. In several animals there were large and/or branching multivesicular bodies. PrPd was associated to membrane invaginations, irregular process profiles, spiral inclusions and lysosomes, but no association was found to vacuoles or to clumped synaptic vesicles.
Discussion: Vacuolation may have more than one cause or morphogenesis. Vacuoles may be caused by the break down of dendritic cytoplasmic content, particularly microtubules, followed by outer membrane ballooning and loss of integrity of the plasmamembrane. Other vacuoles may form due to changes in the membranes of numerous small processes which have an appearance of increased osmiophilia. Cell surface PrPd was associated with an abnormal endocytosis related to cell membrane invaginations which are common in some sheep TSE infections and rare in rodents. Conversely amyloid fibril formations are common in rodents and rare in sheep and cattle TSEs.
AD C. Ersdal, The Norwegian School of Veterinary Science, Department of Basic Sciences and Aquatic Medicine, Norway; C.M. Goodsir, G. McGovern, M. Jeffrey, Veterinary Laboratories Agency, Lasswade Veterinary Laboratory, UK; M.M. Simmons, Veterinary Laboratories Agency, Veterinary Laboratories Agency - Weybridge, UK
SP englisch
PO Schottland