NR AXMA
AU Fasoli,E.; Zanusso,G.; Casalone,C.; Caramelli,M.; Capucci,L.; Monaco,S.
TI Ultrastructural PRP Deposition in BSE and Base Infected Cattle
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.86
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB In 2003 we reported on a novel form of BSE, named bovine amyliodotic spongiform encephaloty (BASE), characterized by the presence of abundant amyloid deposits in the brain. We obtained brain sections from cattles experimentally infected with BSE and BASE. Selected paraffin blocks from olfactory cortices and midbrain were sampled, deparaffinizzated, post-fixed in glutaraldehyde and embedded in Epon. PrP staining was observed on semithin sections etched with potassium methoxide or sodium periodate and incubated with anti-PrP monoclonal antibody SA-65 (previously obtained in our laboratory) after previous treatement with 98% formic acid. We compared the pattern of PrP deposition on paraffin sections with PrP staining in epoxy semithin samples. Although the pattern of PrP accumulation was similar in paraffin and epoxy semithin sections, it maintained distinct characterics in inoculated BSE and inoculated BASE. In fact sections of cerebral cortex of BSE were characterized by a diffuse synaptic pattern with star glial-like deposits of PrP. Instead the cortical sections of BASE showed large PrP aggregates and kuru plaques mainly in the white matter. In midbrain, BSE samples presented a granular intraneuronal and intraxonal pattern of PrP deposition, whereas a perineuronal pattern of prion protein staining was observed in BASE. ICH on semithin section allows a better definition of intraneuronal and glial PrP staining, due to thickness of sections. By analysing strains with different molecular and biological properties, this study provides additional insights on intracellular processing and deposition and on the identification of cells involved in pathological process.
AD E. Fasoli, G. Zanusso, S. Monaco, University of Verona, Italy; C. Casalone, M. Caramelli, IZSPLVA, Italy; L. Capucci, IZSLER, Italy
SP englisch
PO Schottland