NR AXML
AU Foster,J.; Houston,F.; Halliday,S.; McKenzie,C.; Parnham,D.; Drummond,D.; Hunter,N.
TI Does Scrapie Prevent BSE from Replicating and Producing Disease in a Single Host?
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.24
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB There is still the possibility that BSE may have accidentally spread to sheep in the UK national flock and that identifying suspect animals may be obscured if the host is already incubating natural scrapie. This study aims to establish whether it is possible to distinguish BSE and scrapie within a single host or whether the normally distinctive pathological and/or transmissible features of BSE become masked or altered when competing with other TSE sources. This phenomenon akin to strain blocking has been described in murine scrapie models. The study is in two parts. The first is looking at the potential competition between BSE and SSBP/1 following experimental subcutaneous (sc) challenge. To do this New Zealand sheep of the VRQ/ARQ PrP genotype, which are free from natural scrapie, have been injected with SSBP/1, followed three months later by a sc injection of BSE. Other sheep of this genotype have been injected sc with a mixed SSBP/1/BSE inocula. New Zealand sheep of the ARQ/ARQ genotype have also been used and either injected with SSBP/1 followed by one of BSE three months later, or injected with a mixed homogenate of BSE and SSBP/1. The second part of the study is investigating whether NPU sheep predisposed to developing natural scrapie (VRQ/VRQ) can become infected experimentally by sc injection with BSE. Some sheep have been injected at six months of age while other sheep have been injected at eighteen months of age. Sterile material has been recovered from clinically affected sheep in both parts of the study and inoculated into panels of mice as 8 transmissions. Various tissues have been collected for fixation and analysis with several anti-PrP antibodies using immunohistochemistry.
AD J. Foster, C. McKenzie, D. Parnham, D. Drummond, N. Hunter, Roslin Institute/Neuropathogenesis Unit, NPU, UK; F. Houston, S. Halliday, Roslin Institute/Neuropathogenesis Unit/Compton, NPU, UK
SP englisch
PO Schottland