NR AXMN
AU Franscini,N.; El Gedaily,A.; Matthey,U.; Franitza,S.; Zahn,R.
TI Prion Protein in Milk
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.12
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
There is increasing evidence that prions are present in body fluids and that prion infection by blood transmission is possible. Using the technology developed by alicon to detect (alicon PrioMax(R)) and to remove (alicon PrioMin(R)) prion protein, we are able to show that PrPc is present in milk from humans, cows, sheep, and goats1. The absolute amount of PrPc differs between the species, from µg/l range in sheep milk, to ng/l range in human milk. Off-the-shelf milk contains significant amounts of endogenous prion protein even after ultra-high temperature treatment. In view of a recent study showing evidence of prion replication occurring in the mammary gland of scrapie infected sheep suffering from mastitis, the appearance of PrPc in milk implies the possibility that milk of TSE-infected animals serves as a source for PrPsc. The study of the biosafety of milk with regard to TSE has been recently recommended by the European Commission and the European Food Safety Authority.
PrioMax(R) is able to detect minuscule amounts of PrP(R) in milk spiked with brain homogenate. We are currently adapting PrioMax(R) for detection of endogenous PrPsc in milk from scrapie infected sheep. First results may be presented during the conference.
1. Franscini N, Gedaily AE, Matthey U, Franitza S, Sy MS, Burkle A, Groschup M, Braun U, Zahn R. Prion protein in milk. PLoS ONE. 2006 Dec 20;1:e71.
AD N. Franscini, A. El Gedaily, U. Matthey, S. Franitza, R. Zahn, Alicon AG
SP englisch
PO Schottland
EA pdf-Datei und Poster (Das Poster enthält eine Reihe zusätzlicher Autoren und ist identisch mit dem bereits auf der Prion 2006 in Turin gezeigten