NR AXMO
AU Friedman-Levi,Y.; Ovadia,H.; Hoftberger,R.; Abramsky,O.; Budka,H.; Gabizon,R.
TI Scrapie EAE Co-induced Mice, Pathology and Second Generation Transmission
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.53
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB When mice infected with scrapie were induced for EAE, an immune mediated model of CNS inflammation, the co-induced mice died from a distinct progressive neurological disease long before control mice succumbed to classical scrapie. Extensive pathological examinations suggest the co-induced mice suffered from exacerbated inflammation, as seen by increased levels of demyelination, immune cell infiltrates, and gliosis in spinal cords. Interestingly, PrPsc depositions were found in demyelinated white matter areas, suggesting that in scrapie-EAE mice, activated and scrapie infected immune cells may infiltrate into the CNS. As opposed to white matter PrPsc, total brain PrPsc accumulation was similar in high titer experiments between dying co-induced mice and asymptomatic scrapie infected mice sacrificed at the same time. In low titer prion experiments, animals dying of the same co-induced syndrome sometimes presented high levels of brain PrPsc while others did not. Second generation titration experiments suggest that prion titers in the brains of the diverse co-induced mice were independent from their clinical status and related to their levels of PrPsc accumulation. We hypothesize that while inflammatory processes affecting the CNS may have severe clinical implications in subjects incubating prion diseases, such clinical insult did not result in increased infectious potential of the affected animal.
AD Y. Friedman-Levi, H. Ovadia, O. Abramsky, H. Budka, R. Gabizon, Hadassah Hebrew University Medical Centers, Israel; R. Hoftberger, Medical University of Vienna, Austria
SP englisch
PO Schottland
EA pdf-Datei und Poster (zusätzlicher Autor: O. Einstein; Postertitel: Fatal neurological disease in scrapie infected mice induced for EAE)