NR AXMV
AU Gavier-Widen,D.; Ottander,L.; Langeveld,J.; Westergren,E.; Biacabe,A.G.; Baron,T.G.M.
TI Immunohistochemical Features of an H-Type Case of Atypical Bovine Spongiform Encephalopathy in Sweden
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.33
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB Until the date (May 2007), a single case of bovine spongiform encephalopathy (BSE) has been detected in Sweden. It was characterized in tissue homogenates as an unusual BSE case of the H-type based on the molecular features of the protease partially resistant core of the prion protein (PrPres). These included: higher molecular weight of the PrPres bands, affinity to the N-terminus specific monoclonal antibodies (mAbs) 12B2 and P4, and peculiar banding pattern with mAb SAF 84 showing an additional band between 12 14 kDa. The immunohistochemical features were studied on sections of medulla applying mAbs with epitopes covering a broad range of the PrP sequence. The following antibodies were applied: F89/160.1, F99/97.6.1, P4, 12F10, 12B2, 94B4 and R145. All the mAbs resulted in immunostaining but the intensity of different disease associated PrP (PrPd) types varied with the different antibodies. The most frequent PrPd type was thin punctate deposition in the neuropil, sometimes coalescing into coarser granules. This affected the complete grey matter and was most intensive in the lateral aspects of the nucleus of the spinal tract of the trigeminal nerve, followed by the solitary tract nucleus. Perineuronal, and to lesser extent linear, PrPd deposition was also observed. The mAbs F99 and R145 produced the most severe intraneuronal and intramicroglial PrPd depositions, which were also observed, though at a lower level, with mAb 94B4. Accumulation of intraneuronal granules of PrPd was more evident in the large neurons of the reticular formation and of the cuneatus and olivary nuclei. This study describes the epitope recognition of the PrPd in the Swedish H-type case and provides some information of its cellular localization and distribution in the medulla. The authors acknowledge Linda Terry, Veterinary Laboratory Agency, UK, for providing the mAb R145.
AD D. Gavier-Widen, L. Ottander, E. Westergren, National Veterinary Institute (SVA), Sweden; J. Langeveld, CIDC Lelystad, Netherlands; A.G. Biacabe, AFSSALyon, France; T. Baron, AFSSA-Lyon, France
SP englisch
PO Schottland