NR AXMX
AU Gebski,P.; Sikorska,B.; Rieske,P.; Janczar,S.; Liberski,P.P.
TI Normal Prion Protein in Human Placenta
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.174
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
The emergence of variant Creutzfeldt-Jakob disease (vCJD), which is thought to be
caused by consumption of products containing neural tissues form cattle infected with bovine spongiform encephalopathies (BSE), along with recent suggestions that BSE transmission to human may also be linked to sporadic CJD, rises a question about the correct causal attribution of disease. The key event during pathogenesis of transmissible spongiform encephalopathies (TSE) is the conversion of normal prion protein (PrPc) to abnormal, disease-associated isoform PrPSC. Therefore the presence of PrPc in tissues plays important role in TSE pathogenesis. To date, highes levels of PRNP expression have been reported to be found in neural, lymphatic and muscle tissues. Our aim was to search for PrPc in placenta tissue. To achieve that ten placenta tissues and three brain tissues were used in experiment. Western blotting was used to investigate and compare the expression levels of PRNP in both tissues and immunohistochemistry was used to show exact localization of PrPc. All placenta samples used in experiment revealed abundant expression of PRNP with levels of PrPc concentration similar or even higher to those observed in brain samples. PrPc was observed in the syncytiotrophoblast and the cytotrophoblast. This result along with epidemiological data, which supports the possibility of perinatal transmission of the scrapie in sheep, bring up an issue for discussion whether placentas may be involved in spreading CJD in human population, although, yet, neither epidemiological nor pathological evidence seems to confirm that.
AD P. Gebski, B. Sikorska, P. Rieske, S. Janczar, P.P. Liberski, Medical University of Lodz, Poland
SP englisch
PO Schottland