NR AXNH
AU Gonzalez-Romero,D.; Castilla,J.; Morales,R.; Soto,C.
TI Crossing Species Barrier in Vitro by PMCA
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Natural and Experimental Strains P02.21
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB The species barrier phenomenon is defined as the elongation of the incubation period and/or the decrease in the attack rate when prions are transmitted between two different species. In vivo prion adaptation is carried out after several passages until the incubation period is stable, which could take numerous years. In this study we show that species barrier can be crossed in vitro using the PMCA technology. For this purpose we focused on the barrier between mice and hamsters, two well studied experimental models of prion diseases. RML mouse PrPsc was propagated by PMCA using hamster brain homogenate as a source of PrPc. At the same time, 263K hamster PrPsc was propagated in vitro using mouse PrPc. In both cases, the newly generated PrPsc was serially amplified through many rounds of PMCA to remove the initial inoculum. The in vitro generated PrPsc was inoculated into wild type animals leading to the development of a clinically and neuropathologically typical prion disease. Incubation times were similar to mouse-or hamster-adapted prion strains depending on the PrPc source used with no need for strain adaptation. Comparison with existing mouse and hamster strains revealed that the newly generated infectious material possesses some unique biochemical and histopathological features suggesting generation of new prions strains. Our data suggest that the species barrier can be efficiently crossed in vitro by PMCA and indeed we have been able to cross most of the barriers (with some interesting exceptions), even among species in which in vivo experiments have failed. Our findings indicate that PMCA may be a useful methodology to study faster the important phenomenon of species barrier and to assess the infectious and biochemical characteristic of new strains generated after inter-species transmission.
AD D. Gonzalez-Romero, J. Castilla, R. Morales, C. Soto, University of Texas Medical Branch, USA
SP englisch
PO Schottland
EA pdf-Datei und Poster (Autorenliste ergänzt um P. Saa und J. De Castro)