NR AXNN
AU Grießbach,M.; Massag,N.; Baumann,D.; Krex,C.; Biedermann,W.; Truyen,U.; Lücker,E.
TI Differentiation of Species and Age of Central Nervous System Tissue (CNS) for the Detection of SRM
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.94
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
In the wake of the BSE crisis the European Commission released the directive 999/2001 which fixes certain measures to manage the disease. The removal and disposal of Specified Risk Materials (SRM) is the most important task for the protection of the consumer. The main part of the SRM consists of tissues of the central nervous system (CNS) of ruminants (cattle, sheep and goat) older than 12 months of age.
Within the last years, several methods for the detection of CNS have been developed. All these are based on immunochemical or molecular biological analysis of CNSspecific proteins or RNA-sequences. However, all these methods have the same disadvantages: reduction of the detected amount within heat-treated materials and the inability to determine the age of the CNS. Furthermore, only a few markers allow a species identification. Niederer and Bollhalder (2001) chose an alternative approach for the detection of CNS. They suggested the analysis of CNS-specific fatty acids (FA) and calculated a fatty acid ratio (C24:1 w9/w7), which enables the species differentiation. In our work we have improved the given method (Luecker et al. 2004). Further on we could show an excellent stability of the complex lipids, containing CNS-specific FA, to extreme heat conditions (publication in progress). We established new fatty acids and ratios for species differentiation. In addition, we were able to show the age dependence of certain fatty acids, which allow the age categorisation of the CNS (publication in progress).
Overall this method fulfils the requirements for the detection of CNS-based SRM as given by the legal definition.
In this contribution we present the analytical strategy of the GC-MS method for the detection of CNS-based SRM in meat products. We demonstrate the potential differentiation of species and age of the processed CNS by means of specially selected fatty acid ratios.
Niederer M, Bollhalder R. Identification of species specific central nervous tissue by Gas Chromatography -Mass Spectrometry (GC-MS)- a possible method for supervision of meat products and cosmetics. Mitt. Lebensm. Hyg. (2001) 92: 133-144
Luecker E. et al. GC-MS detection of central nervous tissue as TSE risk material in meat products: analytical quality and strategy. Anal Bioanal Chem (2004) 380: 866-870
AD M. Grießbach, C. Krex, W. Biedermann, E. Lücker, Institute of Food Hygiene, Germany; N. Massag, Novartis Pharma AG, Switzerland; D. Baumann, U. Truyen, Institute of Animal Hygiene and Veterinary Public Health, Germany
SP englisch
PO Schottland