NR AXOK
AU Herva,M.E.; Espinosa,J.C.; Andreoletti,O.; Padilla,D.; Alamillo,E.; Lacroux,C.; Torres,J.M.
TI Selective Susceptibility of Porcine species to Prion Strains as Assayed in Transgenic Mice Expressing Porcine PrP
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Natural and Experimental Strains P02.29
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
Background: There have been no reports of naturally occurring TSE in porcine species. Nevertheless, pigs are susceptible to BSE infection but showing a strong transmission barrier by parenteral route. Previous work in our group using transgenic mice expressing porcine PrP (PoPrP-Tg001) showed a strong transmission barrier for BSE by intracerebral route.
Objectives: The main aim of this work was to characterise the susceptibility of porcine species to different prion strains in transgenic mice expressing porcine PrP Tg mice.
Methods: A wide panel of inocula has been used as intracerebral inoculum in PoPrPTg001: BSE, RML, different human and sheep TSEs.
Results: From the different strains assayed, only the BSE prion strain has been able to be transmitted to mice. Remarkably, BSE after passage in sheep increased its transmission ability in PoPrP-Tg001 mice that is maintained after subpassage.
Discussion: Our results confirm that porcine species shows an strong transmission barrier to a wide range of prion strains but the risk of infection of BSE after passage in sheep need to be considered.
AD M.E. Herva, J.C. Espinosa, D. Padilla, E. Alamillo, J.M. Torres, CISA-INIA, Spain; O. Andréoletti, C. Lacroux, INRA-ENVT, France
SP englisch
PO Schottland