NR AXOM
AU Hesp,J.R.; Kirby,E.; Dickinson,J.; Dennis,M.; Cornwall,M.; Raven,N.D.H.; Sutton,J.M.
TI Development of a Standardised Approach to Assess the Effectiveness of Current and New Decontamination Technologies against TSE Agents
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.101
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
Background: The development of inactivation methods for Transmissible spongiform encephalopathies (TSEs) is an urgent requirement in relation to the potential for iatrogenic transmission of variant Creutzfeldt Jakob Disease (vCJD). The evaluation of the effectiveness of such methodologies requires a highly sensitive and specific assay or a combination of assays. With current cellular and biochemical based assays still in development, the bioassay remains the accepted approach to assess effectiveness; however, careful matching between the TSE strain and host species is required to help ensure that the risks are appropriately evaluated with regard to vCJD transmission.
Aims: The project aims to develop a robust system to assess proposed TSE inactivation technologies focusing on a model using the TSE strain, BSE-301V, designed to mimic the key features of possible vCJD transmission via contaminated surgical instruments. The dynamic range of the model was determined using a titration series of infectivity which in the first instance was 'tested' using a conventional autoclave based process.
Methods. BSE-301V infected mouse brain homogenate, previously titrated to x109 ID50 per gram, was dried onto the surface of surgical steel suture wires using a standardised process. Wires were implanted i.c. into VM mice and monitored for clinical symptoms for up to 550 days.
Results: For the wire-based titration series clinical symptoms were observed in animals from groups across a 6-log dilution range, however, at dilutions below 10-3 transmission rates fell below 60%, suggesting that the useful range is around 4-logs. Data will be presented comparing the surface bound titration results with the equivalent in-solution titration series. The ongoing results from the decontamination studies will also be presented in relation to the titration data generated.
Conclusions: Methods have been established to ensure a consistent exposure of wires to the decontamination process with no further manipulations of the carriers post processing. Using this protocol a titration series has been established for BSE-301V on surgical steel that potentially covers a 4-log range. The use of these protocols to evaluate novel prion decontamination methods will be discussed.
AD J.R. Hesp, E. Kirby, J. Dickinson, M. Dennis, M. Cornwall, N.D.H. Raven, J.M. Sutton, Health Protection Agency, Research, UK
SP englisch
PO Schottland