NR AXOS
AU Hortells,P.; Monleon,E.; Acin,C.; Bolea,R.; Vargas,A.; Vasseur,V.; Ryffel,B.; Badiola,J.J.; Monzon,M.
TI Consequences of Copper and Manganese Imbalances on the Scrapie Progress
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.28
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB Environmental exposure to some metals is thought to explain the occurrence of sporadic Transmissible Spongiform Encephalopathy (TSEs); however, the published results are not conclusive. The present study is focused on the assessment of the effect of copper depletion and/or manganese enhancement in diet on susceptibility to Scrapie as well as on its progress. A total of 118 prion protein deficient (PrP0/0) and transgenic (Tg20) mice were divided into different groups according to metal altered regimens. Animals were inoculated by intraperitoneal injection, also including matched controls in all groups. In addition to the incubation period, the severity of neuropathological changes was assessed in all animals. Infection was detected in 100% of Tg20 mice whereas no PrPsc was observed in any PrP0/0. Spongiosis in all of those receiving a copper depleted diet was the highest, but it was also found in noninfected animals. These observations suggest this diet as the main responsible for this lesion and for its worsening in the inoculated animals. The highest intensities of glial fibrillary acidic protein (GFAP) immunostaining were also associated with the copper depleted diet. Dietary manganese supplementation was demonstrated to have a negative effect on neuronal counts. In conclusion, this study demonstrates that certain environmental factors could aggravate the neurological Scrapie lesions, as reported with regard to other neurodegenerative diseases where some metalloenzymes plays a pivotal protector role against the oxidative stress.
AD P. Hortells, E. Monleón, C. Acín, R. Bolea, A. Vargas, J.J. Badiola, M. Monzón, University of Zaragoza, Research Centre for Prion Diseases, Spain; V. Vasseur, B. Ryffel, Centre National de la Recherche Scientifique, France
SP englisch
PO Schottland