NR AXOT
AU Hortells,P.; Monleon,E.; Acin,C.; Bolea,R.; Vargas,A.; Vasseur,V.; Ryffel,B.; Badiola,J.J.; Monzon,M.
TI Effect of the Dimethoate Administration on a Scrapie Murine Model
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.48
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB Some authors have connected organophosphate compounds to Transmissible Spongiform Encephalopathy (TSE) susceptibility. Nevertheless, the actual role played by these compounds still remains unclear. The aim of the study presented was to assess the effect of oral exposure of dimethoate (DMT) on Scrapie susceptibility as well as on the progress of the disease by using a genetically modified murine model. A total of 48 lacking (PrP0/0) and 70 over-expressing PrP gene (Tg20) C57BL/6 mice were included in the present study. A portion of them was intraperitoneally (IP) inoculated, whilst the rest was maintained as non infected control. Specifically, they were inoculated in 78 cases and the remaining 40 were used as non-inoculated control mice. Exposure to DMT dissolved in drinking water since the beginning of the experiment in the corresponding cases (no control) was performed. The incubation period, spongiosis, PrPsc deposits, glial over-expression (GFAP), neuronal loss (NeuN) and amyloid plaques were the variables which were assessed in all animals. According to the results provided, a treatment consisting of a daily 15 mg/Kg dose of DMT for 5 weeks did not show any effect on any of the variables assessed either on any of the transgenic lines probed. Despite more exhaustive studies for assessing different doses and organic compounds are required, this finding constitutes an empirical study that rules out the possibility that this compound may have a predisposing effect on TSEs.
AD P. Hortells, E. Monleón, C. Acín, R. Bolea, A. Vargas, J.J. Badiola, M. Monzón, University of Zaragoza, Research Centre for Prion Diseases, Spain; V. Vasseur, B. Ryffel, Centre National de la Recherche Scientifique, France
SP englisch
PO Schottland