NR AXOU
AU Howlin,R.P.; Pinchin,H.; McDonnell,G.; Keevil,B.
TI Assessment of Prion Decontamination Procedures on Surgical Stainless Steel Surfaces
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.92
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
Recently we have developed a rapid, sensitive staining procedure for in situ detection of prion amyloid material on surgical stainless steel surfaces using fluorescent thiazole reagents. Initial data show that this procedure is several fold more sensitive than conventional Western blot detection of equivalent infected tissue that has not been first dried onto instrument surfaces. In addition, recognised inefficiency in elution of the amyloid off the steel surfaces will further reduce the sensitivity of the Western blot assay compared to the in situ staining procedure.
The stain employs a thiazole incubation in addition to a SYPRO Ruby step to detect both amyloid and general tissue protein simultaneously in situ on surgical stainless steel surfaces. This procedure is currently being used to assess various cleaning chemistries from several companies for their ability to remove or inactivate PrPsc. However, in reality, a range of several different detergents or enzymatic cleaning reagents may be used within automated washer-disinfector cycles. As such, we have utilized our dual stain to assess the effects of different pre-treatments and cleaning reagents on prion removal throughout the course of validated washer-disinfector cycles. This work is being performed in collaboration with the CEA, Paris who have an established animal model of infectivity to confirm the efficacy of the disinfection procedures. This collaboration will allow the new rapid, dual stain method to be developed for in situ detection of PrPsc and general protein contamination on surgical steel surfaces and its suitability to assess prion removal (hence disinfection) during SSD cleaning procedures.
AD R.P. Howlin, H. Pinchin, B. Keevil, University of Southampton, School of Biological Sciences, UK; G. McDonnell, Steris Ltd, UK
SP englisch
PO Schottland