NR AXPD
AU Jang,B.; Kim,E.; Choi,J.K.; Jin,J.K.; Ishigami,A.; Kim,Y.S.; Choi,E.K.
TI Increased Citrullinated Proteins in The Brain of Scrapie-Infected Mice
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.14
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB Prion diseases are neurodegenerative disorders by a pathogenic isoform of the prion protein (PrPsc). Although the key event in the pathology of prion diseases is thought to be the conversion of cellular prion protein (PrPc) to the protease-resistant pathogenic form (PrPsc), the factors that contribute to neurodegeneration in scrapie-infected animals are poorly understood. It can be suggested that there are possibilities of structural differences or differential post-translational modification between PrPc and PrPsc. Citrullination is a post-translational modification process that arginine residue in protein convert to citrulline in a calcium(Ca2+)-dependent condition by peptidylarginine deiminases (PADs). PAD2 is widely expressed type in various tissues and a unique expressed type in brain. It has been reported that the increased citrullinated proteins were observed in degenerative disorders. To elucidate the involvement of protein citrullination in prion diseases, we examined whether citrullinated proteins are produced in the brains of scrapie-infected mice. PAD2 immunoreactivity and its expression levels of both mRNA and protein were significantly increased in scrapieinfected brains and PAD2 was mainly detected in reactive astrocytes. Supporting this data, the activity of PAD2 in scrapie-infected brains was significantly increased compared to control brains. We have also found that citrullinated proteins of varied molecular weights were detected in both control and scrapie-infected brains and that the level of citrullinated proteins increased in the brains of scrapie-infected mice. This study suggests that PAD2 has an important role in the pathogenesis of prion diseases and that the citrullinated proteins may be involved in the pathogenesis of prion diseases.
AD B. Jang, E. Kim, J.K. Choi, J.K. Jin, Y.S. Kim, E.K. Choi, Hallym University, Ilsong Institute of Life Science, Korea; A. Ishigami, Tokyo Metropolitan Institute of Gerontology, Molecular Pathology, Japan
SP englisch
PO Schottland