NR AXPM
AU Julius,C.; Yusa,S.; Parkyn,C.; Mootoosamy,R.; Morris,R.; Campenot,B.; Glatzel,M.; Miele,G.; Aguzzi,A.
TI Neuronal Propagation of Prions
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.78
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB Prions are the causative agents of Transmissible Spongiform Encephalopathies (TSEs). In vivo experiments suggest an involvement of the peripheral nervous system in prion spread to the brain after peripheral exposure to prions, but detailed knowledge about intranerval prion propagation remains elusive. Here we show uptake of fluorescently labeled Scrapie Associated Fibrils (SAFs) by primary cultures derived from Superior Cervical Ganglia (SCGs). Furthermore we employ a compartmented culture system which allows the investigation of prion propagation by physically separating the liquid environment of cell bodies and neurite endings of SCGs. We exposed neurites to scrapie prions for four weeks and detected prion infectivity in cell bodies. We also inoculated cell bodies with prions and detected prion infectivity in the neurites. Hence, applying this system we show anterograde as well as retrograde intraneural transport of prion infectivity in peripheral neurons without the involvement of accessory cells. Since prion diseases are ultimately fatal, understanding the basic principles of prion transport could lead to strategies blocking prion spread after peripheral uptake and therefore help in the abatement of prion disease.
AD C. Julius, S. Yusa, A. Aguzzi, University Hospital Zürich, Switzerland; C. Parkyn, R. Mootoosamy, R. Morris, Wolfson Centre for Age-Related Diseases, UK; B. Campenot, University of Alberta, Canada; M. Glatzel, University Hospital of Hamburg-Eppendorf, Germany; G. Miele, University of Dundee, UK
SP englisch
PO Schottland
EA pdf-Datei und Poster (Posterautoren: C. Julius, C. Sigurdson, N. Zeller, S. Yusa, C. Parkyn, R. Mootoosamy, R. Morris, R.B. Campenot, M. Glatzel, G. Miele und A. Aguzzi; Postertitel: Prion Spread Occurs Retrograde and Anterograde in Peripheral Neurons)