NR AXQI
AU Kranich,J.; Zabel,M.D.; Heikenwalder,M.; Arrighi,I.; Schwarz,P.; Prinz,M.; von Teichman,A.; Haas,K.M.; Zeller,N.; Tedder,T.F.; Weis,J.H.; Aguzzi,A.
TI CD21/35 - A Peripheral Prion Receptor?
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.64
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB The immune system plays a central role in prion diseases by providing reservoirs for prion accumulation and replication. Follicular dendritic cells (FDCs) efficiently replicate and accumulate prions in lymphoid compartments, presumably by using antigentrapping molecules of the complement system. However, the exact mechanism how prions are captured and targeted to FDCs is still unknown. Mice deficient of the complement receptor CD21/35, which is expressed by FDCs and B cells show drastically prolonged incubation times. By reciprocal bone marrow reconstitutions with wild-type and CD21/35-/-mice we could show that CD21/35-expression by radiation resistant FDCs and not by radio sensitive B cells is crucial for peripheral prion replication. Furthermore, immunoprecipitation experiments using the anti-PrP antibody POM2 followed by elution of the PrP-complex with epitope-mimicking peptides showed that CD21/35 is co-precipitated with PrPsc but not with PrPc. This indicates that CD21/35 directly binds PrPsc and therefore aids prion replication on FDCs. Whether opsonization of PrPsc by soluble complement factors is required for binding to CD21/35 is currently under investigation.
AD
J. Kranich, M. Heikenwalder, I. Arrighi, P. Schwarz, A. von Teichman, N. Zeller, A. Aguzzi, University Hospital Zürich, Switzerland; M. Zabel, Colorado State University College of Veterinary Medicine and Biomedical Sciences, USA; M. Prinz, Georg-August Universität, Germany;
K.M. Haas, T.F. Tedder, Duke University Medical Center, USA; J.H. Weis, University of Utah, USA
SP englisch
PO Schottland
EA pdf-Datei und Poster (Posterautoren: J. Kranich, M.D. Zabel, I. Arrighi, P. Schwarz, A. von Teichman, K.M. Haas. N. Zeller, M. Heikenwalder, T.F. Tedder, J.H. Weis und A. Aguzzi; Postertitel: CD21: The peripheral prion receptor?)