NR AXQL
AU Krex,C.; Grießbach,M.; Franziska,H.; Biedermann,W.; Baumann,D.; Truyen,U.; Lücker,E.
TI Validation of a Reference Method for the Detection of CNS in Meat Products by Means of GC/MS
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.97
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
In the course of the mad cow crisis several measures were established in certain member states, and finally, Europe wide. These measures aimed at a reduction of the human exposure risk with the agent of Transmissible Spongiform Enzephalopathies (TSE). The highest priority has the removal and harmless disposal of Specified Risk Materials (SRM) to this day. The legal definition of SRM according to the European directive 999/2001 defines tissues of concern, in particular the central nervous system (CNS: brain and spinal cord) from cattle, sheep and goats over 12 months of age.
Methods for the detection of CNS that are currently available are based on a more or less uniform immunochemical principle. They only detect tissues of the CNS without further information on species and age of the animal and therefore differ from the given legal definition of SRM. This applies to molecular biological techniques and speciesspecific immunoassays since these methods do not identify the age of the CNS as well. Moreover, false negative results or at least less precise quantification of the CNS are to be expected due to heat processing effects on the used markers during production of meat products or meat and bone meal (MBM).
In the course of a research project, which was funded by the Federal Ministry of Food, Agriculture and Consumer Protection (BMELV) a procedure using gas chromatography mass spectrometry (GC/MS) was developed and optimized. On the basis of CNStypical fatty acids and their patterns, the detection of the CNS including their age and species was possible additionally to the quantification of the CNS. The complex lipids, containing these fatty acids where shown to be even thermally stable up to extreme conditions not reached during food and feedstuff processing. Currently, this method is being evaluated in a ring test organised by the Institute of Food Hygiene, University of Leipzig. This project is funded by the BMELV.
The present contribution summarises first results on the detection of CNS in meat products and MBM from the ongoing ring test.
AD C. Krex, M. Grießbach, H. Franziska, W. Biedermann, E. Lücker, Institute of Food Hygiene, Germany; D. Baumann, U. Truyen, Institute of Animal Hygiene and Veterinary Public Health, Germany
SP englisch
PO Schottland