NR AXQR
AU Ladhani,K.; Minor,P.; Cooper,J.K.
TI Preparation of Standard Materials from Cases of BSE and Scrapie
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.85
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
In collaboration with the VLA (Weybridge) a unique bank of tissue and blood samples have been collected from animals suspected of incubating BSE or Scrapie. Tissues collected include spleen and brain (areas predicted to contain high levels of PrPsc) and were prepared as 10% (or 5%) homogenates; where possible tissues have been evaluated for PrPsc content.
Genotypes were determined by sequence analysis of amplified PRNP DNA. Whole blood was collected into citrate (CPD) buffer and separated into plasma, buffy coat and red cell components using protocols and reagents that would most closely mimic those used by the National Blood Service (NBS).
To date, samples have been collected from 3 confirmed clinical cases of scrapie and a control animal. Bovine blood and tissues have been collected from 2 clinical and 6 suspect BSE cases. Diagnosis was confirmed by the VLA. This collection is part of an increasing bank of reagents held by NIBSC (www.nibsc.ac.uk/cjd), to be used in the development of TSE diagnostic assays. Animal samples are distributed subject to the Independent Archive Advisory Group (IAAG) approval. This work was funded by DEFRA grant SD0426
AD K. Ladhani, P. Minor, J. Cooper, NIBSC, CJD Resource Centre, UK
SP englisch
PO Schottland