NR AXQX
AU Lasmezas,C.; Lescoutra,N.; Comoy,E.; Holznagel,E.; Loewer,J.; Motzkus,D.; Hunsmann,G.; Ingrosso,L.; Bierke,P.; Pocchiari,M.; Ironside,J.; Will,R.; Deslys,J.P.
TI Interim Transmission Results in Cynomolgus Macaques Inoculated with BSE and vCJD Blood Specimens
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Oral Abstracts FC5.1.2
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Vortrag
AB
BSE and vCJD transmitted to cynomolgus macaques reproduce many features of human vCJD, including clinical symptoms, neuropathological hallmarks of vCJD, PrPres electrophoretical pattern and, most importantly, the wide distribution of infectivity in peripheral organs. The latter characteristic distinguishes vCJD from sCJD in both humans and cynomolgus macaques, and prompted us to use this non-human primate model for further investigations of vCJD and its risk for human health. The occurrence of four vCJD infections in humans transfused with blood from patients who later developed vCJD has raised concern about blood transfusion safety in countries with vCJD.
In this collaborative European study, we investigated the infectivity of blood components and whole blood administered by intracerebral (ic) and intravenous (iv) routes. Buffy-coat and whole blood was inoculated by ic and iv route, respectively, from two vCJD patients and from two clinical vCJD-inoculated macaques. Transfusions were also performed from whole blood and blood leucodepleted according to hospital practice standards from two clinical BSE inoculated macaques. Blood infectivity during the preclinical phase is being examined in orally infected macaques. Whole blood was collected and transfused from one such animal two years after oral challenge, whereas buffy-coat and plasma from two animals at 2 and 4.5 years post-challenge, respectively, have been inoculated by the ic route.
This is an ongoing study in which recipient animals continue to be observed at various times post-inoculation. So far, we have had one positive transmission in one animal transfused 65 months earlier with 40 ml of whole blood from a vCJD macaque (the characteristics of the disease in this animal will be shown in a separate poster by E. Comoy). This positive transmission reproduces transfusion transmission of vCJD in humans, with an incubation of 5.5 years compatible with incubation periods observed in humans.
AD C. Lasmezas, Scripps Florida, Infectology, USA; N. Lescoutra, E. Comoy, J.P. Deslys, CEA, France; E. Holznagel, J. Loewer, PEI, Germany; D. Motzkus, G. Hunsmann, DPZ, Germany; L. Ingrosso, M. Pocchiari, Istituto Superiore di Sanita, Italy; P. Bierke, SMI, Sweden; J. Ironside, R. Will, CJD Surveillance Unit, UK
SP englisch
PO Schottland