NR AXSF
AU Marshall,A.S.; Manson,J.C.; Winton,D.J.; Clarke,A.R.; Tuzi,N.L.
TI Investigating the Effect of Conditional Expression of PrP Protein in the Intestine upon TSE Pathogenesis
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.23
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB The host PrP protein is essential for the establishment of transmissible spongiform encephalopathy (TSE) diseases. Although PrP is expressed at high levels in the central nervous system, it is also expressed in a wide variety of peripheral cells. Oral ingestion of TSE infected material is thought to be the most common route in most natural forms of TSE disease, however, the details of how 'infectivity' crosses the gut epithelial barrier and enters the nervous system following oral exposure are not yet fully understood. Therefore the aims of this project are to investigate the requirement of PrP expression in intestinal epithelial cells and enteric neurones in oral infection. To achieve this we have used our conditional transgenic mouse lines (generated using Cre-LoxP techniques) that possess floxed PrP genes. By crossing these animals with transgenic mice possessing inducible intestinal epithelial cell specific or enteric neurone specific Cre recombinase we are able to generate mouse models with restricted PrP expression in these cell types. We have shown the induction of Cre recombinase activity in the intestinal epithelial cell mouse model to produce excellent recombination in the villi of the intestine. Studies are now underway to infect these animals orally with TSE agents to determine the effect of removal of PrP expression from these cells on TSE disease.
AD A.S. Marshall, J.C. Manson, N.L. Tuzi, Neuropathogenesis Unit, Roslin Institute, UK; D.J. Winton, Cancer Research UK, Cambridge Institute for Medical Research, Addenbrooke's Hospital, Department of Oncology, UK; A.R. Clarke, School of Biosciences, University of Cardiff, UK
SP englisch
PO Schottland