NR AXSI
AU Masujin,K.; Wells,G.A.H.; Matthews,D.; Iwamaru,Y.; Imamura,M.; Matsuura,Y.; Shimizu,Y.; Okada,H.; Mohri,S.; Yokoyama,T.
TI Prions in Peripheral Nervous System Tissues in Bovine Spongiform Encephalopathy (BSE)
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.39
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB It has been shown that the abnormal isoform of prion protein (PrPsc) is not limited to the specified risk materials in bovine spongiform encephalopathy (BSE). We investigated PrPsc distribution by a highly sensitive western blotting (WB) in tissues from naturally and experimentally BSE-infected cattle. In natural BSE, confirmed by PrPsc detection at the medulla - obex, PrPsc was detected in autonomic and somatic components of the peripheral nerve system (PNS), including certain cranial nerves. To determine whether parts of the PNS, other than those implicated directly in the hypothetical pathogenetic spread of BSE prions from the intestine to the central nervous system (CNS), become involved before or after the CNS is affected, we investigated PrPsc distribution by WB in dorsal root ganglia, stellate ganglion, phrenic, radial, and sciatic nerves and adrenal, relative to detection of PrPsc in CNS of cattle orally inoculated with BSE affected brain and culled sequentially. In experimental BSEinfected cattle, PrPsc was first detected in dorsal root ganglia concurrent with PrPsc in the CNS and only subsequently in the other tissues tested. PrPsc was confirmed in the adrenals of cattle that showed clinical signs. No PrPsc was detected in the PNS of BSE-negative cattle. This study shows that, with respect to dorsal root ganglia, a paravertebral sympathetic ganglion and the somatic nerves examined, PrPsc is detected inconsistently in the PNS during the disease course, coincident with, or after it accumulates in the CNS and is potentially widespread in PNS in natural cases at the time of diagnosis.
AD K. Masujin, Y. Iwamaru, M. Imamura, Y. Matsuura, Y. Shimizu, H. Okada, S. Mhori, T. Yokoyama, National Institute of Animal Health, Japan; G.A.H. Wells, D. Matthews, Veterinary Laboratory Agency, UK
SP englisch
PO Schottland